:::Development of betulinic acid as an agonist of TGR5 receptor using a new in vitro assay.
Development of betulinic acid as an agonist of TGR5 receptor using a new in vitro assay. 員工報告
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| 計畫摘要 | "BACKGROUND: G-protein-coupled bile acid receptor 1, also known as TGR5 is known to be involved in glucose homeostasis. In animal models, treatment with a TGR5 agonist induces incretin secretion to reduce hyperglycemia. Betulinic acid, a triterpenoid present in the leaves of white birch, has been introduced as a selective TGR5 agonist. However, direct activation of TGR5 by betulinic acid has not yet been reported. METHODS: Transfection of TGR5 into cultured Chinese hamster ovary (CHO-K1) cells was performed to establish the presence of TGR5. Additionally, TGR5-specific small interfering RNA was employed to silence TGR5 in cells (NCI-H716 cells) that secreted incretins. Uptake of glucose by CHO-K1 cells was evaluated using a fluorescent indicator. Amounts of cyclic adenosine monophosphate and glucagon-like peptide were quantified using enzyme-linked immunosorbent assay kits. RESULTS: Betulinic acid dose-dependently increases glucose uptake by CHO-K1 cells transfected with TGR5 only, which can be considered an alternative method instead of radioligand binding assay. Additionally, signals coupled to TGR5 activation are also increased by betulinic acid in cells transfected with TGR5. In NCI-H716 cells, which endogenously express TGR5, betulinic acid induces glucagon-like peptide secretion via increasing calcium levels. However, the actions of betulinic acid were markedly reduced in NCI-H716 cells that received TGR5-silencing treatment. Therefore, the present study demonstrates the activation of TGR5 by betulinic acid for the first time. CONCLUSION: Similar to the positive control lithocholic acid, which is the established agonist of TGR5, betulinic acid has been characterized as a useful agonist of TGR5 and can be used to activate TGR5 in the future." |
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| 資料集研究(主辦)機關 | 衛生局聯合醫院 |
| 年度 | 105 |
| 研究人員 | Lo SH(羅士翔), Cheng KC, Li YX, Chang CH, Cheng JT, Lee KS. |
| 關鍵詞 | 期刊 |
| 備註 | 本專區110年以前為歷史轉檔資料，因新舊平臺欄位規格不同，故有部分詮釋資料欄位無資訊；如對資料內容有疑義，請洽資料集主辦機關或本府研考會研究發展組。 |